Quantitative Determination and Characterization of a Kashmir Saffron (<i>Crocus sativus</i> L.)-Based Botanical Supplement Using Single-Laboratory Validation Study by HPLC-PDA with LC–MS/MS and HPTLC Investigations
Aboli Girme, Ganesh Saste, Sandeep Pawar, Chetana Ghule, Amit Mirgal, Saurabh Patel, Anshuly Tiwari, Sautik Ghoshal, Sandip B. Bharate, Sonali S. Bharate, D. Srinivasa Reddy, Ram A. Vishwakarma, Lal Hingorani
Abstract
= 2) was calculated and found as 0.0035-0.007% (<5.0%) as per the EURACHEM guide for this HPLC analysis. Additionally, an untargeted identification of 36 compounds in the botanical supplement was based on the elution order, UV-vis spectra, mass fragmentation pattern, and standards by ESI-MS/MS analysis with comprehensive chromatographic fingerprinting. Thus, these analytical approaches enable a composite profile of the stigma-based extract as a potential supplement for human health benefits.
Topics & Concepts
Crocus sativusChromatographyHigh-performance liquid chromatographyGradient elutionChemistryTraditional medicineMedicineSaffron Plant Research StudiesFlavonoids in Medical ResearchPhytochemistry and Biological Activities