Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli
Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae‐Yean Kim
Abstract
Dual base editors are DSB-free CRISPR tools applied in eukaryotes but not yet in bacteria. We developed an improved ACBE toolset for bacteria, combining highly processive deaminases. We believe that the bacterial optimized iACBE toolset is a significant advancement in CRISPR-based E. coli genome editing and adaptable to other microbes.
Topics & Concepts
Escherichia coliCytosineBacteriaDual (grammatical number)ChemistryBase (topology)Computational biologyMicrobiologyBiochemistryBiologyDNAGeneticsPhilosophyMathematicsGeneMathematical analysisLinguisticsCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsBacterial Genetics and Biotechnology