<i>Clostridioides difficile</i> TcdB Toxin Glucosylates Rho GTPase by an S<sub>N</sub><i>i</i> Mechanism and Ion Pair Transition State
Ashleigh S. Paparella, Sean M. Cahill, Briana L. Aboulache, Vern L. Schramm
Abstract
mechanism with the formation of a distinct oxocarbenium phosphate ion pair transition state where the glycosidic bond to the UDP leaving group breaks prior to attack of the threonine nucleophile from Rho GTPase.
Topics & Concepts
ChemistryGTPaseClostridium difficile toxin BOxocarbeniumGlucosyltransferaseLeaving groupStereochemistryBiochemistryClostridium difficile toxin ANucleophileEnzymeClostridium difficileCatalysisAntibioticsClostridium difficile and Clostridium perfringens researchToxin Mechanisms and Immunotoxins