The colony forming efficiency assay for toxicity testing of nanomaterials—Modifications for higher-throughput
Elise Rundén‐Pran, Espen Mariussen, Naouale El Yamani, Elisabeth Elje, Eleonora Longhin, Mária Dušinská
Abstract
toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with the read-out or the reagent of the assay needs to be addressed to avoid biased results. Thus, validated label-free methods are urgently needed for hazard identification of NMs to avoid unintended adverse effects on human health. The colony forming efficiency (CFE) assay is a label- and interference-free method for quantification of cytotoxicity by cell survival and colony forming efficiency by CFE formation. The CFE has shown to be compatible with toxicity testing of NMs. Here we present an optimized protocol for a higher-throughput set up.
Topics & Concepts
ThroughputToxicityNanomaterialsHigh-throughput screeningProtocol (science)Hazard analysisCytotoxicityChemistryComputational biologyNanotechnologyComputer scienceIn vitroBiologyReliability engineeringMedicineMaterials scienceEngineeringBiochemistryTelecommunicationsWirelessOrganic chemistryAlternative medicinePathologyNanoparticles: synthesis and applications3D Printing in Biomedical ResearchCell Image Analysis Techniques