Rapid and Accurate Detection of Aminoglycoside-Modifying Enzymes and 16S rRNA Methyltransferases by Targeted Liquid Chromatography-Tandem Mass Spectrometry
Dimard E. Foudraine, Nikolaos Strepis, Corné H. W. Klaassen, Merel N. Raaphorst, Annelies Verbon, Theo M. Luider, Wil H. F. Goessens, Lennard J. M. Dekker
Abstract
= 191). The category interpretations were correctly predicted for gentamicin in 97.4% of the isolates, for tobramycin in 97.4% of the isolates, and for amikacin in 82.7% of the isolates. Targeted LC-MS/MS can be applied for accurate and rapid detection of aminoglycoside resistance mechanisms.
Topics & Concepts
MethyltransferaseAminoglycosideChromatographyTandem mass spectrometryMass spectrometryRibosomal RNAChemistryBiologyBiochemistryAntibioticsMethylationDNAGeneGenomics and Phylogenetic StudiesAntibiotic Resistance in BacteriaBacterial Identification and Susceptibility Testing