New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses
Rafael Salto, María D. Girón, Virginia Puente‐Muñoz, José D. Vilchez, Laura Espinar‐Barranco, Javier Valverde-Pozo, Daniele Arosio, José M. Paredes
Abstract
isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations.
Topics & Concepts
ChlorideChromophoreFluorescenceChemistryIsomerizationIntracellularMutagenesisFluorescent proteinBiophysicsPhotochemistryBiochemistryGreen fluorescent proteinBiologyOrganic chemistryCatalysisMutationQuantum mechanicsGenePhysicsPhotoreceptor and optogenetics researchNeuroscience and Neuropharmacology ResearchAdvanced Fluorescence Microscopy Techniques