Predictive high-throughput screening of PEGylated lipids in oligonucleotide-loaded lipid nanoparticles for neuronal gene silencing
Apoorva Sarode, Yuchen Fan, Amy E. Byrnes, Michal Hammel, Greg L. Hura, Yige Fu, Ponien Kou, Chloe Hu, Flora I. Hinz, Jasmine Roberts, Stefan G. Koenig, Karthik Nagapudi, Casper C. Hoogenraad, Tao Chen, Dennis Leung, Chun‐Wan Yen
Abstract
efficacy. The reported HTS workflow can be used to screen additional multivariate parameters of LNPs with significant time and material savings, therefore guiding the selection and scale-up of optimal formulations for nucleic acid delivery to a variety of cellular targets.
Topics & Concepts
PEG ratioGene silencingChemistryPEGylationHigh-throughput screeningBiophysicsIn vitroOligonucleotideBiochemistryPolyethylene glycolGeneBiologyFinanceEconomicsRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesMicroRNA in disease regulation