Amorphous RuO <sub>2</sub> Nanozymes with an Excellent Catalytic Efficiency Superior to Natural Peroxidases
Zirui Qin, Lei Jiao, Lijun Hu, Yiming Zhang, Xiangkun Jia, Chengjie Chen, Xiaolei Sun, Yanling Zhai, Zhijun Zhu, Xiaoquan Lu
Abstract
Abstract Developing efficient peroxidase‐like nanozymes to surpass natural enzymes remains a significant challenge. Herein, an amorphous RuO 2 nanozyme with peroxidase‐like activity is synthesized for activating H 2 O 2 with a specific activity of 1492.52 U mg −1 , outperforming the crystalline RuO 2 nanozymes by a factor of 22 and far superior to natural peroxidases. Amorphous RuO 2 nanozymes with long‐range disordered atomic arrangements can effectively elongate the O─O bonds in H 2 O 2 . Abundant oxygen vacancies in amorphous RuO 2 nanozymes lead to an upshift of the d ‐band center, enhancing the exceptional adsorption strength of H 2 O 2 , which improve the electron transfer efficiency and ensure superior peroxidase‐like activity. Accordingly, a nanozyme‐linked immunosorbent assay is developed for the precise and sensitive detection of prostate‐specific antigens with a detection limit as low as 0.52 pg mL −1 . This study introduces a simple approach for developing high‐performance peroxidase‐like nanozymes to improve the analytical performances of prostate‐specific antigens in clinical diagnostics.