In situ observation of mitochondrial biogenesis as the early event of apoptosis
Changsheng Shao, Xiuhong Zhou, Yuhui Miao, Peng Wang, Qianqian Zhang, Qing Huang
Abstract
detection of mitochondrial fluorescence changes, we also examined the cell viability, nuclear DNA damage, reactive oxygen species (ROS), mitochondrial superoxide, citrate synthase activity, ATP, cytoplasmic and mitochondrial calcium, mitochondrial mass, mitochondrial morphology, and protein expression related to mitochondrial biogenesis, as well as the apoptosis biomarkers. As a result, we confirmed that significant mitochondrial biogenesis took place preceding the radiation-induced apoptosis, and it was closely correlated with the apoptotic cells at late stage. The involved mechanism was also discussed.
Topics & Concepts
In situBiogenesisEvent (particle physics)ApoptosisMitochondrial biogenesisMitochondrionChemistryCell biologyComputational biologyBiophysicsBiologyPhysicsBiochemistryGeneOrganic chemistryQuantum mechanicsMitochondrial Function and PathologyCell death mechanisms and regulationAdipose Tissue and Metabolism