Bifidobacterium canis sp. nov., a novel member of the Bifidobacterium pseudolongum phylogenetic group isolated from faeces of a dog (Canis lupus f. familiaris)
Věra Bunešová, Gabriele Andrea Lugli, Nikol Modráčková, Marie Makovska, Jakub Mrázek, Chahrazed Mekadim, Šárka Musilová, Ivona Svobodová, Roman Špánek, Marco Ventura, Jiří Killer
Abstract
A fructose-6-phosphate phosphoketolase-positive strain (GSD1FS T ) was isolated from a faecal sample of a 3 weeks old German Shepherd dog. The closest related taxa to isolate GSD1FS T based on results from the EZBioCloud database were Bifidobacterium animalis subsp. animalis ATCC 25527 T , Bifidobacterium animalis subsp. lactis DSM 10140 T and Bifidobacterium anseris LMG 30189 T , belonging to the Bifidobacterium pseudolongum phylogenetic group. The resulting 16S rRNA gene identities (compared length of 1454 nucleotides) towards these taxa were 97.30, 97.23 and 97.09 %, respectively. The pairwise similarities of strain GSD1FS T using argS , atpA , fusA , hsp 60, pyrG , rpsC , thrS and xfp gene fragments to all valid representatives of the B. pseudolongum phylogenetic group were in the concatenated range of 83.08–88.34 %. Phylogenomic analysis based on whole-genome methods such as average nucleotide identity revealed that bifidobacterial strain GSD1FS T exhibits close phylogenetic relatedness (88.17 %) to Bifidobacetrium cuniculi LMG 10738 T . Genotypic characteristics and phylogenetic analyses based on nine molecular markers, as well as genomic and comparative phenotypic analyses, clearly proved that the evaluated strain should be considered as representing a novel species within the B. pseudolongum phylogenetic group named as Bifidobacterium canis sp. nov. (GSD1FS T =DSM 105923 T =LMG 30345 T =CCM 8806 T ).