Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor.
Salahadin Bahrami, Amir Amiri‐Yekta, Abbas Daneshipour, Seyedeh Hoda Jazayeri, Paul Mozdziak, Mohammad Hossein Sanati, Hamid Gourabi
Abstract
Specific developmental characteristics of the chicken make it an attractive model for the generation of transgenic organisms. Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection. Culturing chicken primordial germ cells (PGCs) and their ability for germline transmission ushered in a new stage in this regard. With the advent of CRISPR/Cas9 system, a new phase of studies for manipulating genomes has begun. It is feasible to integrate a desired gene in a predetermined position of the genome using CRISPR/Cas9 system. In this review, we discuss the new approaches and technologies that can be applied to generate a transgenic chicken with regards to recombinant protein productions.