A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
Rose Ruiz Daniels, Richard S. Taylor, Ross Dobie, Sarah J. Salisbury, James Furniss, Emily Clark, Daniel J. Macqueen, Diego Robledo
Abstract
The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.
Topics & Concepts
NucleusProtocol (science)Computational biologyBiologyIsolation (microbiology)Extraction (chemistry)Computer scienceEvolutionary biologyBioinformaticsChemistryCell biologyChromatographyMedicineAlternative medicinePathologyEnvironmental DNA in Biodiversity StudiesFish Ecology and Management StudiesMicrobial Community Ecology and Physiology