Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy
Jonathan A. Stefely, Yu Zhang, Elyse C. Freiberger, Nicholas W. Kwiecien, Hala Elnakat Thomas, Alexander M. Davis, Nathaniel D. Lowry, Catherine E. Vincent, Evgenia Shishkova, Nicholas A. Clark, Mario Medvedovic, Joshua J. Coon, David J. Pagliarini, Carol A. Mercer
Abstract
ATG: autophagy-related; CALCOCO1: calcium binding and coiled-coil domain protein 1; CALCOCO2/NDP52: calcium binding and coiled-coil domain protein 2; CLIR: MAP1LC3C-interacting region; CQ: chloroquine; KO: knockout; LIR: MAP1LC3-interacting region; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MEF: mouse embryonic fibroblast; MLN: MLN0128 ATP-competitive MTOR kinase inhibitor; MTOR: mechanistic target of rapamycin kinase; reticulophagy: selective autophagy of the endoplasmic reticulum; TAX1BP1/CALCOCO3: TAX1 binding protein 1; ULK: unc 51-like autophagy activating kinase; WT: wild-type.