Pathogen-Specific Bactericidal Method Mediated by Conjugative Delivery of CRISPR-Cas13a Targeting Bacterial Endogenous Transcripts
Zihao Song, Yue Yu, Xinpeng Bai, Yiguo Jia, Jiayi Tian, Kui Gu, Mengyu Zhao, Changyu Zhou, Xiangyu Zhang, Hongning Wang, Yizhi Tang
Abstract
Antibiotics with broad-spectrum activities are known to disturb both pathogens and beneficial gut microbiota and cause many undesired side effects, prompting increased interest in developing therapies that specifically eliminate pathogenic bacteria without damaging gut resident flora. To achieve this goal, we developed a strategy utilizing bacterial conjugation to deliver CRISPR-Cas13a programmed to specifically kill S. Typhimurium.
Topics & Concepts
CRISPRSalmonella entericaBiologyMicrobiologyPathogenAntibioticsSalmonellaEscherichia coliBacteriaComputational biologyGeneticsGeneCRISPR and Genetic EngineeringVibrio bacteria research studiesBacterial biofilms and quorum sensing