Janthinobacterium violaceinigrum sp. nov., Janthinobacterium aquaticum sp. nov. and Janthinobacterium rivuli sp. nov., isolated from a subtropical stream in China
Huibin Lu, Tongchu Deng, Zhipeng Cai, Feifei Liu, Xunan Yang, Yonghong Wang, Meiying Xu
Abstract
Four strains assigned the names FT13W T , FT14W, FT58W T and FT68W T were isolated from a subtropical stream in PR China. All the strains were Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile with flagella. Comparisons based on 16S rRNA gene sequences showed that strains FT13W T , FT14W, FT58W T and FT68W T belonged to genus Janthinobacterium and shared 16S rRNA gene similarities in the range of 98.8–99.7 % with Janthinobacterium lividum DSM 1522 T , Janthinobacterium agaricidamnosum DSM 9628 T and ‘ Janthinobacterium svalbardensis JA-1', respectively. The calculated pairwise average nucleotide identity (ANI) values among the genomes of above seven strains were in the range of 79.0–92.2 %, except that the ANI value was 96.8 % between strain FT13W T and FT14W. The respiratory quinone of strains FT13W T , FT14W, FT58W T and FT68W T was determined to be Q-8. The major fatty acids were C 16 : 1 ω7 c , C 16 : 0 , C 18 : 1 ω7 c and C 12 : 0 . The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The genome sizes of strains FT13W T , FT14W, FT58W T and FT68W T were 6.45, 6.38, 5.73 and 6.37 Mbp with G+C contents of 63.4, 63.7, 61.6 and 63.1 mol%, respectively. Combining phenotypic, biochemical, genotypic and ANI data, strain FT13W T and FT14W should belong to the same species. The four strains were considered to represent three novel species within genus Janthinobacterium , for which the names Janthinobacterium violaceinigrum sp. nov. (type strain FT13W T =GDMCC 1.1638 T =KACC 21319 T ), Janthinobacterium aquaticum sp. nov. (FT58W T =GDMCC 1.1676 T =KACC 21468 T ) and Janthinobacterium rivuli sp. nov. (FT68W T =GDMCC 1.1677 T =KACC 21469 T ) are proposed.