AlphaFold2 predicts the inward‐facing conformation of the multidrug transporter LmrP
Diego del Alamo, Cédric Govaerts, Hassane S. Mchaourab
Abstract
As part of the CASP competition, the protein structure prediction algorithm AlphaFold2 generated multiple models of the proton/drug antiporter LmrP. Previous distance restraints from double electron-electron resonance spectroscopy, a technique which reports distance distributions between spin labels attached to proteins, suggest that one of the lower-ranked models may have captured a conformation that has so far eluded experimental structure determination.
Topics & Concepts
CASPAntiporterTransporterProtein structure predictionChemistrySpectroscopyCrystallographyProtein structureComputational biologyBiophysicsAlgorithmPhysicsBiological systemNuclear magnetic resonanceComputer scienceBiologyBiochemistryGeneMembraneQuantum mechanicsElectron Spin Resonance StudiesAdvanced NMR Techniques and ApplicationsMass Spectrometry Techniques and Applications