Steam-exploded <i>Dictyophora indusiata</i> polysaccharide protect against inflammation by regulating gut barrier, microbiota and metabolites
Zhi-Kun Zeng, Lin Wang, Zihao Zhang, Ya-Qing Lin, Bin Xiong, Baodong Zheng, Yi Zhang, Lei Pan
Abstract
The present study aimed to investigate the effects of steam-exploded <i>Dictyophora indusiata</i> polysaccharide (DIPS) on lipopolysaccharide (LPS)-induced inflammation of mice. The results showed that DIPS significantly improved colonic shortening by LPS-induced inflammation, and the mRNA expression of Occludin (<i>Occ</i>) and Zonula occludens-1 (<i>ZO-1</i>) in colon tissues was increased 9.86- and 2.69-fold after DIPS intervention, respectively. In addition, DAI scores decreased from 1.15 to 0.74, while the mRNA expression of pro-inflammatory cytokines tumor necrosis factor-α (<i>TNF-α</i>) and interleukin-6 (<i>IL-6</i>) were also reduced by DIPS intervention. It is remarkable that DIPS modulated the composition of gut microbiota and enhanced the relative abundance of <i>Bifidobacterium</i>, unclassified_o_<i>Oscillospirales</i> and <i>Faecalibaculum</i>. Furthermore, the levels of acetic acid and propionic acid of the gut contents in mice were restored to (0.94 ± 0.24) mg/g and (0.23 ± 0.10) mg/g after the intervention of DIPS, which was 3.03- and 2.56-fold of the LPS group, respectively. In conclusion, DIPS protected against LPS-induced inflammation, which provides new insights into the anti-inflammatory effect of edible fungi polysaccharide.