Brief guide to Caenorhabditis elegans imaging and quantification
Hyemin Min, Gyutae Park, Seung‐Jae Lee
Abstract
Caenorhabditis elegans is a powerful model organism for fluorescence imaging to study gene regulation and cellular biomarkers. This concise MiniResource introduces essential imaging techniques for live and fixed C. elegans, sample preparations, microscopy, and common applications. We also outline basic image analysis workflows using open-source software. This guideline serves as a practical resource for researchers who seek implementing fluorescence imaging with C. elegans.
Topics & Concepts
Caenorhabditis elegansComputational biologyBiologyChemistryGeneticsGeneCell Image Analysis TechniquesGenetics, Aging, and Longevity in Model OrganismsSingle-cell and spatial transcriptomics