Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity
Mark Shaaya, Jordan Fauser, Anastasia Zhurikhina, Jason E. Conage‐Pough, Vincent Huyot, Martin Brennan, Cameron T. Flower, Jacob Matsche, Shahzeb Khan, Viswanathan Natarajan, Jalees Rehman, Pradeep Kota, Forest M. White, Denis Tsygankov, Andrei V. Karginov
Abstract
Engineered allosteric regulation of protein activity provides significant advantages for the development of robust and broadly applicable tools. However, the application of allosteric switches in optogenetics has been scarce and suffers from critical limitations. Here, we report an optogenetic approach that utilizes an engineered Light-Regulated (LightR) allosteric switch module to achieve tight spatiotemporal control of enzymatic activity. Using the tyrosine kinase Src as a model, we demonstrate efficient regulation of the kinase and identify temporally distinct signaling responses ranging from seconds to minutes. LightR-Src off-kinetics can be tuned by modulating the LightR photoconversion cycle. A fast cycling variant enables the stimulation of transient pulses and local regulation of activity in a selected region of a cell. The design of the LightR module ensures broad applicability of the tool, as we demonstrate by achieving light-mediated regulation of Abl and bRaf kinases as well as Cre recombinase.