Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs
Katherine E. Bowden, Sandeep J. Joseph, John C. Cartee, Noa Ziklo, Damien Danavall, Brian H. Raphael, Timothy D. Read, Deborah Dean
Abstract
Chlamydia trachomatis is an obligate intracellular bacterium that is not easily cultured, which limits our understanding of urogenital and rectal C. trachomatis transmission and impact on morbidity. To provide a publicly available workflow for whole-genome target enrichment and sequencing of C. trachomatis directly from clinical urine, vaginal, conjunctival, and rectal specimens, we developed and report on an RNA bait library to enrich the organism from clinical samples for sequencing.
Topics & Concepts
Chlamydia trachomatisObligateGenitourinary systemBiologyWhole genome sequencingGenomeChlamydiaceaeChlamydiaChlamydia trachomatis infectionLymphogranuloma venereumDNA sequencingMicrobiologyChlamydialesVirologyDNAImmunologyGeneticsGeneAnatomyEcologyReproductive tract infections researchUrinary Tract Infections ManagementGut microbiota and health