Topical ripasudil stimulates neuroprotection and axon regeneration in adult mice following optic nerve injury
Euido Nishijima, Kazuhiko Namekata, Atsuko Kimura, Xiaoli Guo, Chikako Harada, Takahiko Noro, Tadashi Nakano, Takayuki Harada
Abstract
Optic nerve injury induces optic nerve degeneration and retinal ganglion cell (RGC) death that lead to visual disturbance. In this study, we examined if topical ripasudil has therapeutic potential in adult mice after optic nerve crush (ONC). Topical ripasudil suppressed ONC-induced phosphorylation of p38 mitogen-activated protein kinase and ameliorated RGC death. In addition, topical ripasudil significantly suppressed the phosphorylation of collapsin response mediator protein 2 and cofilin, and promoted optic nerve regeneration. These results suggest that topical ripasudil promotes RGC protection and optic nerve regeneration by modulating multiple signaling pathways associated with neural cell death, microtubule assembly and actin polymerization.