Efficient generation of mouse models with the prime editing system
Yao Liu, Xiangyang Li, Siting He, Shuhong Huang, Chao Li, Yulin Chen, Zhen Liu, Xingxu Huang, Xiaolong Wang
Abstract
Most genetic diseases in humans are caused by single-nucleotide mutations. Although genome editing with either the CRISPR-based cytosine base editor (CBE) 1 or the adenine base editor (ABE) 2 holds great promise for gene correction of C-to-T and A-to-G base substitutions in some genetic diseases 3 , 4 , both editors are useless for correction of other variants such as base transversion, small insertions and deletions (indels).
Topics & Concepts
Prime (order theory)Computer scienceChemistryComputational biologyBiologyMathematicsCombinatoricsTeaching and Learning ProgrammingEvolutionary Algorithms and Applications