Associations of Gut Microbiota and Fatty Metabolism With Immune Thrombocytopenia
Xiao-Min Yu, Qingyun Zheng, Yun He, Dandan Yu, Guolin Chang, Cheng Chen, Laixi Bi, Jia Lv, Misheng Zhao, Xiangyang Lin, Liqing Zhu
Abstract
Objective To determine whether gut microbiota, fatty metabolism and cytokines were associated with immune thrombocytopenia (ITP). Methods In total, 29 preliminarily diagnosed ITP patients and 33 healthy volunteers were enrolled. Fecal bacterial were analyzed based on 16S rRNA sequencing. Plasma cytokines and motabolites were analyzed using flow cytometry and liquid chromatography-mass spectrometry (LC-MS), respectively. Results Bacteroides , Phascolarctobacterium , and Lactobacillus were enriched at the genus level in ITP patients, while Ruminococcaceae UCG-002 , Eubacterium coprostanoligeues , Megamonas, and Lachnospiraceae NC2004 were depleted. At the phylum level, the relative abundance of Proteobacteria and Chloroflexi increased in ITP patients, while Firmicutes , Actinobacteria , and the Firmicutes/Bacteroidetes ratio decreased. Plasma levels of 5-hydroxyeicosatetraenoic acid (5-HETE), 6-trans-12-epi-leukotriene B4 (6t,12e-LTB 4 ), and resolvin D2 (RvD 2 ) were upregulated, and stachydrine, dowicide A, dodecanoylcarnitine were downregulated in ITP patients. Furthermore, RvD 2 is positively correlated with order Bacteroidetes VC2.1 Bac22 , 5-HETE is positively correlated with genus Azospirillum , and 6t,12e-LTB 4 is positively correlated with genus Cupriavidus . In addition, stachydrine is positively correlated with family Planococcaceae , dowicide A is positively correlated with class MVP-15 , and dodecanoylcarnitine is positively correlated with order WCHB1-41 . Plasma levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were upregulated in ITP patients. Conclusion Our study revealed a relationship between microbiota and fatty metabolism in ITP. Gut microbiota may participate in the pathogenesis of ITP through affecting cytokine secretion, interfering with fatty metabolism.