Engineering the activity of amine dehydrogenase in the asymmetric reductive amination of hydroxyl ketones
Hui Ming, Bo Yuan, Ge Qu, Zhoutong Sun
Abstract
An engineered AmDH derived from a leucine dehydrogenase was used as the starting enzyme to improve its activity in the synthesis of ( R )-3-amino-1-butanol. Preparative-scale synthesis of the ( R )-product (90% yield, >99%) was performed on a gram-scale.
Topics & Concepts
Reductive aminationYield (engineering)AminationChemistryAmine gas treatingCombinatorial chemistryCatalysisOrganic chemistryDehydrogenaseEnzymeMaterials scienceMetallurgyEnzyme Catalysis and ImmobilizationPharmacogenetics and Drug MetabolismChemical Reactions and Isotopes