Progenitor cell diversity in the developing mouse neocortex
Xiangbin Ruan, Bowei Kang, Qi Cai, Wenhe Lin, Jingshu Wang, Xiaochang Zhang
Abstract
Significance How projection neuron types are temporally and sequentially generated in the mammalian neocortex remains unclear. We performed single-cell RNA sequencing analysis of embryonic day (E) 10.5 through E18.5 mouse neocortical cells and identified progenitor cell types across development. Our results uncovered molecular signatures for neuroepithelial cells and temporal gene expression in radial glial progenitors. Importantly, Eomes -positive cells display temporal expression of previously characterized neuronal identity genes. These results delineate neocortical progenitor cell diversity and the timing of neuron type specification.
Topics & Concepts
NeocortexProgenitor cellBiologyNeuroepithelial cellProgenitorEmbryonic stem cellCell typeNeural stem cellCell biologyNeuronNeuroscienceCellStem cellGeneGeneticsSingle-cell and spatial transcriptomicsNeuroinflammation and Neurodegeneration MechanismsNeurogenesis and neuroplasticity mechanisms