Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress
Michael Hagemann-Jensen, Christoph Ziegenhain, Rickard Sandberg
Abstract
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughput. Smart-seq3xpress analysis of peripheral blood mononuclear cells resulted in a granular atlas complete with common and rare cell types. Compared with droplet-based single-cell RNA sequencing that sequences RNA ends, the additional full-transcript coverage revealed cell-type-associated isoform variation.
Topics & Concepts
RNASingle cell sequencingSingle-cell analysisCellComputational biologyGene isoformBiologyRNA-SeqPeripheral blood mononuclear cellTranscriptomeCell biologyGene expressionGeneGeneticsMutationExome sequencingIn vitroSingle-cell and spatial transcriptomicsExtracellular vesicles in diseaseCancer Genomics and Diagnostics