Single-cell ATP detection and content analyses in electrostimulus-induced apoptosis using functionalized glass nanopipettes
Dandan Wang, Guohua Qi, Ya Zhou, Ying Zhang, Bo Wang, Ping Hu, Yongdong Jin
Abstract
A glass nanopipette functionalized with ATP-responsive gold nanoparticle assemblies was developed for ATP detection in single-cells and used for analysing the content change of ATP during electrostimulus (ES)-induced apoptosis. The variation of ATP content in single normal cells and cancer cells during apoptosis was detected by the method.
Topics & Concepts
ApoptosisAdenosine triphosphateCancer cellCellBiophysicsChemistryMaterials scienceNanotechnologyCell biologyBiochemistryCancerBiologyGeneticsAdvanced biosensing and bioanalysis techniquesNanopore and Nanochannel Transport StudiesRNA Interference and Gene Delivery