Aloperine-Type Alkaloids with Antiviral and Antifungal Activities from the Seeds of <i>Sophora alopecuroides</i> L.
Zhan‐Xing Hu, Ji Zhang, Tong Zhang, Cai-Yan Tian, Qiao An, Ping Yi, Chun‐Mao Yuan, Zhongkai Zhang, Lihua Zhao, Xiao‐Jiang Hao
Abstract
As a continuous flow investigation of novel pesticides from natural quinolizidine alkaloids, the chemical compositions of the seeds of Sophora alopecuroides were thoroughly researched. Fifteen new aloperine-type alkaloids ( 1 – 15 ) as well as six known aloperine-type alkaloids ( 16 – 21 ) were obtained from the extract of S. alopecuroides . The structures of 1 – 21 were confirmed via HRESIMS, NMR, UV, IR, ECD calculations, and X-ray diffraction. The antiviral activities of 1 – 21 against tobacco mosaic virus (TMV) were detected following the improved method of half-leaf. Compared with ningnanmycin (protective: 69.7% and curative: 64.3%), 15 exhibited excellent protective (71.7%) and curative (64.6%) activities against TMV. Further biological studies illustrated that 15 significantly inhibited the transcription of the TMV- CP gene and increased the activities of polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL). The antifungal activities of 1 – 21 against Phytophythora capsica, Botrytis cinerea, Alternaria alternata, and Gibberella zeae were screened according to a mycelial inhibition test. Compound 13 displayed excellent antifungal activity against B. cinerea (EC 50: 7.38 μg/mL). Moreover, in vitro antifungal mechanism studies displayed that 13 causes accumulation of reactive oxygen species and finally leads to mycelia cell membrane damage and cell death in vitro .