Effect of hydrogen sulfide (H2S) on the growth and development of tobacco seedlings in absence of stress
Jingcheng Dai, Dingxin Wen, Hao Li, Jingpeng Yang, Xiongfei Rao, Yong Yang, Jiangke Yang, Chunlei Yang, Jun Yu
Abstract
Abstract Background Hydrogen sulfide (H 2 S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H 2 S in promoting the growth and development of tobacco plants is still unclear. Results In this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0 mM of sodium hydrosulfide (NaHS) on endogenous H 2 S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H 2 S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0 mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis. Conclusions These results would aid in enhancing our understanding of the involvement of H 2 S, a novel signaling molecule to promote the growth and development of tobacco plants.