Highly sensitive and specific fluorescence biosensor for global 5–hydroxymethylcytosine detection based on M.HhaI methyltransferase catalysis and the improved isothermal exponential amplification reaction
Yaqian An, Yaping Li, Xuelian Chen, Lirong Han, Xuelian Xin, Hailiang Nie, Xian Zhang, Cuiping Li
Topics & Concepts
Detection limitDNAChemistryFluorescenceLoop-mediated isothermal amplificationMethyltransferaseLinear rangeCombinatorial chemistryMolecular biologyBiophysicsBiochemistryBiologyChromatographyMethylationQuantum mechanicsPhysicsEpigenetics and DNA MethylationAmino Acid Enzymes and Metabolism