Evidence for low-level translation in human erythrocytes
Sangeetha Devi Kumar, Debaleena Kar, Md Noor Akhtar, Belinda Willard, Debadrita Roy, Tanweer Hussain, Purusharth I Rajyaguru, Sandeep M. Eswarappa
Abstract
(β-globin) transcripts are translated. Using a luciferase-based reporter assay and mutational studies, we show that the sequence of the 5' untranslated region is crucial for the translation of these transcripts. Furthermore, mature erythrocytes showed reduced expression of globin proteins (α- and β-) when treated with translation inhibitors. Overall, we provide multiple lines of evidence for translation of globin mRNAs in human mature erythrocytes.
Topics & Concepts
PolysomeBiologyGlobinTranslation (biology)Protein biosynthesisMolecular biologyMethionineRibosomeMessenger RNARNAUntranslated regionCell biologyBiochemistryGeneAmino acidErythrocyte Function and PathophysiologyNanopore and Nanochannel Transport StudiesRNA and protein synthesis mechanisms