Human liver afferent and efferent nerves revealed by 3-D/Airyscan super-resolution imaging
Chien‐Chia Chen, Shih-Jung Peng, Ya-Hsien Chou, Chih‐Yuan Lee, Po‐Huang Lee, Rey‐Heng Hu, Ming‐Chih Ho, Mei‐Hsin Chung, Fu-Ting Hsiao, Yu‐Wen Tien, Shiue–Cheng Tang
Abstract
We embed the human liver (vs. pancreas, positive control) in the high-refractive-index polymer for tissue clearing and antifade 3-D/Airyscan super-resolution neurohistology. The pancreas-liver comparison reveals: 1) sensory nerves in the hepatoportal space; 2) intralobular sympathetic innervation, including the nerve fibers and varicosities contacting the ballooning hepatocytes; and 3) parasympathetic innervation of the septal bile duct. Our results highlight the sensitivity and resolving power of 3-D/Airyscan super-resolution imaging in human liver neurohistology.
Topics & Concepts
EfferentPathologyAnatomyAutofluorescenceBiologyChemistryMedicineAfferentPhysicsQuantum mechanicsFluorescenceNeuroscience and Neuropharmacology ResearchNeurogenesis and neuroplasticity mechanismsBiochemical effects in animals