Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
Kathryn A. Murray, Ruben M. F. Tomás, Matthew I. Gibson
Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
Topics & Concepts
CryopreservationCryoprotectantMesenchymal stem cellRegenerative medicineFlow cytometryStromal cellCell biologyCell therapyStem cellChemistryBiologyImmunologyCancer researchEmbryoMesenchymal stem cell researchTissue Engineering and Regenerative MedicineElectrospun Nanofibers in Biomedical Applications