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Molecular characterization and genome sequencing of selected highly resistant clinical isolates of Pseudomonas aeruginosa and its association with the clustered regularly interspaced palindromic repeat/Cas system

Hekmat A. Owaid, Mushtak T.S. Al-Ouqaili

2025Heliyon29 citationsDOIOpen Access PDF

Abstract

represent a potential threat to public health and contribute to the seriousness of associated illnesses by leading to resistant infections. Further, WGS for the two strains revealed resistance to multiple antibiotics. It is important to examine specific antimicrobial resistance (AMR) pathways, which suggests that a significant number of resistant genes in these isolates indicate a loss of CRISPR genes in the two strains. Furthermore, the WGS approach can lead to a better understanding of the genomic mechanism of pseudomonal resistance to antibiotics.

Topics & Concepts

Pseudomonas aeruginosaPalindromeBiologyGeneticsCRISPRDNA sequencingWhole genome sequencingGenomePalindromic sequenceComputational biologyDNAGeneBacteriaAntibiotic Resistance in BacteriaBacterial biofilms and quorum sensingBacteriophages and microbial interactions