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Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

Benedetta Artegiani, Delilah Hendriks, Joep Beumer, Rutger N.U. Kok, Xuan Zheng, Indi P. Joore, Susana M. Chuva de Sousa Lopes, Jeroen S. van Zon, Sander J. Tans, Hans Clevers

2020Nature Cell Biology286 citationsDOIOpen Access PDF

Topics & Concepts

CRISPRGenome editingOrganoidCas9Gene knockinComputational biologyBiologyGenomeCell biologyGenome engineeringGeneticsGeneCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchChromosomal and Genetic Variations
Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing | Litcius