Regulation of inorganic polyphosphate is required for proper vacuolar proteolysis in fission yeast
Naoya Sawada, Shiori Ueno, Kojiro Takeda
Abstract
-deficient cells was mediated by the polyphosphate synthase VTC complex in vacuoles. Simultaneous deletion of VTC complex subunits rescued Pqr1 mutant phenotypes, including defects in proteolysis and loss of viability during quiescence. We conclude that excess polyphosphate may interfere with proteolysis in vacuoles by mechanisms that as yet remain unknown. The present results demonstrate a connection between polyphosphate metabolism and vacuolar functions for proper autophagy-dependent proteolysis, and we propose that polyphosphate homeostasis contributes to maintenance of cellular viability during quiescence.
Topics & Concepts
PolyphosphateProteolysisVacuoleSchizosaccharomyces pombeCell biologyBiologyAutophagyBiochemistryProteasomeLysosomeUbiquitinUbiquitin ligaseCytosolSaccharomyces cerevisiaeMutantYeastPhosphateGeneEnzymeApoptosisCytoplasmFungal and yeast genetics researchPolyamine Metabolism and ApplicationsCellular transport and secretion