Detection of the HIV-1 Accessory Proteins Nef and Vpu by Flow Cytometry Represents a New Tool to Study Their Functional Interplay within a Single Infected CD4 <sup>+</sup> T Cell
Jérémie Prévost, Jonathan Richard, Romain Gasser, Halima Medjahed, Frank Kirchhoff, Beatrice H. Hahn, John C. Kappes, Christina Ochsenbauer, Ralf Duerr, Andrés Finzi
Abstract
HIV-1 Nef and Vpu exert several biological functions that are important for viral immune evasion, release, and replication. Here, we developed a new method allowing simultaneous detection of these accessory proteins in their native form together with some of their cellular substrates. This allowed us to show that Vpu cannot compensate for the lack of a functional Nef, which has implications for studies that use Nef-defective viruses to study ADCC responses.
Topics & Concepts
Antibody-dependent cell-mediated cytotoxicityBiologyCell biologyFlow cytometryVirologyEpitopeViral replicationImmune systemIntracellularAntibodyVirusMonoclonal antibodyMolecular biologyGeneticsHIV Research and TreatmentImmune Cell Function and InteractionT-cell and B-cell Immunology