A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury
Peng He, Mi Zhang, Meng Zhao, Mengyao Zhang, Benxu Ma, Hongyu Lv, Yantao Han, Ding‐Tao Wu, Zhangfeng Zhong, Wenwen Zhao
Abstract
We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum , namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe 2+ level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.