Occurrence, Antimicrobial Resistance, and Virulence of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa Isolated from Dairy Products
Walaa G. Nadi, Ibrahim Lamiaa, Abdel Ahmed, Eman M. Awad, Taher, Abd Halem, S, L Ahmed, Ibrahim, Abdel Salam, A Fahim, K, W Ahmed, A Samar, W Ahmed, I Helal, M Zaky, H Abdulla, Al-Ashmawy Ma, K Sallam, S Abd-Elghany, M Elhadidy, T Tamura, H Al-Talib, C Yean, A Al-Khateeb, H Hassan, Kkb Singh, Al-Jashamy K Ravichandran, M, Ama Ammar, Ssa El-Shafii, Amo, Abd El, -Dayim Zae, R Atia, H Mohamed, Abo Elroos, N, Awad Dab, M Bahar, S Jamali, A Samadikuchaksaraei, M Bandyopadhyay, B Chakraborty, S Kumar, R Ghosh, M Chatterjee, P Mukhopadhyay, M Bandyopadhyay, M Brian, M Frosolono, B Murray, A Miranda, E Lopez, H Gomez, T Cleary, R Castro, Shsp Pedroso, Shc Sandes, G Silva, Kcm Luiz, R Dias, Rat Filho, Hcp Figueiredo, S Santos, A Nunes, M Souza, G Catho, R Martischang, F Boroli, M Chrati, Y Martin, Koyluk Tomsuk, Z Renzi, G Schrenzel, J Pugin, J Nordmann, P Blanc, D Harbarth, S, Disease Centers For, Prevention Control, F Dehkordi, F Yazdani, J Mozafari, Y Valizadeh, El Bagoury, A Shelaby, H Saied, H, A Eleboudy, A Amer, M Nasief, S Eltony, M Elhadidy, M Mohammed, R El-Mahdy, G El-Kannishy, H Fadel
Abstract
One of the significant hurdles in the 21 st century is the contamination of dairy products with pathogenic and spoilage microorganisms that convey antimicrobial-resistant genes (AMR).Therefore, this study examined the dominance of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli in 60 samples of white soft cheese and Baladi yoghurt (30 of each).Phenotypic resistance to ten antimicrobials was assessed with molecular characterization of mecA and blaIMP genes in S. aureus and P. aeruginosa, respectively.Additionally, slt1, slt2, and eaeA virulence genes were ascertained in the identified E. coli isolates.S. aureus, P. aeruginosa, and E. coli were present in 19, 15%; 51, 40%; and 1, 3% of the isolated strains in white soft cheese and Baladi yoghurt, respectively.S. aureus (n=34) isolated strains were insensitive phenotypically to methicillin, yet only 77% carried the mecA gene.While the identified P. aeruginosa strains were unsusceptible to the studied antibiotics except for meropenem, and the blaIMP gene was found in the molecularly identified strains.The molecularly identified E. coli (n=3) were sensitive to imipenem and meropenem.Additionally, Slt1 and Slt2 virulence genes were present in all E. coli isolates, whereas 66% of them possessed the eaeA gene.The application of systems of food safety and Good Manufacturing Practices (GMP) is essential to ensure the safety and quality of commercial dairy products.