Hemolysin BL from novel <i>Bacillus toyonensis</i> BV-17 induces antitumor activity both in vitro and in vivo
Jiajia Chen, Shoukui Hu, Dengbo Ji, Zhaoya Gao, Hanyang Wang, Yong Yang, Yongkang Chen, Jin Gu
Abstract
BV-17 cultures could quickly kill various tumor cell lines within minutes in vitro, by causing cell membrane disruption, blebbing, and leakage of cytoplasmic content. Fast protein liquid chromatography (FPLC) and mass spectrometry analysis identified hemolysin BL (HBL) as the effector molecule, which exhibits a different cytotoxicity mechanism compared to previous studies. Intra-tumor injection of low dose HBL inhibited the growth of both treated and untreated tumors in mice. The outcomes of this pioneer study suggest that HBL exhibits antitumor activity and is a potential chemotherapeutic agent that could be engineered to target only tumor cells in future.