Base editing‐mediated targeted evolution of ACCase for herbicide‐resistant rice mutants
Hongzhi Wang, Yuxin He, Yingying Wang, Zuren Li, Jiannan Hao, Yijiao Song, Mugui Wang, Jian‐Kang Zhu
Abstract
Improved cytosine and adenine base editors and an efficient dual editor were applied in targeted evolution of ACETYL COA CARBOXYLASE in rice, resulting in the generation of dozens of herbicide-resistant mutations, at least three of which, W2125L, W2125Q and C2186H, have not been reported previously.
Topics & Concepts
MutantCytosineBase (topology)Rice plantBiologyGeneticsDNAAgronomyGeneMathematicsMathematical analysisCRISPR and Genetic EngineeringPlant Virus Research StudiesInsect Resistance and Genetics