Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells
Fenghua Zhang, Xianmei Li, Mudan He, Ye Ding, Feng Xiong, Golpour Amin, Zuoyan Zhu, Yonghua Sun
Topics & Concepts
CRISPRZebrafishCas9BiologyGenome editingMaternal to zygotic transitionMutantGeneticsGeneZygoteGene knockoutCell biologyEmbryogenesisCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchAnimal Genetics and Reproduction