Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
Shiela Chetri, Bhaskar Jyoti Das, Deepshikha Bhowmik, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee
Abstract
OBJECTIVE: The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. RESULTS: 12 isolates were found over-expressing AcrAB-TolC efflux pump system and showed reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, over expression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.
Topics & Concepts
RegulonEffluxPorinMeropenemMicrobiologyEscherichia coliBacterial outer membraneGeneBiologyGene expressionCarbapenemAntibioticsAntibiotic resistanceGeneticsAntibiotic Resistance in BacteriaAntibiotics Pharmacokinetics and EfficacyPharmaceutical and Antibiotic Environmental Impacts