Dihydro-Si-rhodamine for live-cell localization microscopy
Xiaodong Zhang, Mengmeng Zhang, Yan Yu, Mingkang Wang, Jin Li, Yang Yu, Yi Xiao, Xiao Luo, Xuhong Qian, Youjun Yang
Abstract
Fluorophores with photo-modulatory fluorescence properties are valuable for cutting-edge localization microscopy. The existing probes are either photo-activatable, or photo-switchable, but not both. We report a probe (DH-SiR), a leuco-dye obtained by reduction of Si-rhodamine, with both photo-activatable and photo-switchable fluorescence. The potential for super-resolution microscopy was showcased.
Topics & Concepts
MicroscopyRhodamineRhodamine BChemistryFluorescence microscopeNanotechnologyMaterials scienceBiophysicsFluorescenceBiologyOpticsPhysicsBiochemistryCatalysisPhotocatalysisAdvanced Fluorescence Microscopy Techniquesbioluminescence and chemiluminescence researchLuminescence and Fluorescent Materials