Activity of Fusarium oxysporum-Based Silver Nanoparticles on Candida spp. Oral Isolates
Maísa Santos Fonseca, Daniela Méria Rodrigues, Ana Rita Sokolonski, Danijela Stanišić, Luíz Marcelo Ribeiro Tomé, Aristóteles Goés‐Neto, Vasco Azevedo, Roberto Meyer, Danilo Barral de Araújo, Ljubica Tasić, Ricardo Wagner Portela
Abstract
Candida spp. resistant to commercially available antifungals are often isolated from patients with oral candidiasis, a situation that points to the need for the development of new therapies. Thus, we evaluated the activity of Fusarium oxysporum-based silver nanoparticles (AgNPs) on Candida spp. isolated from denture stomatitis lesions. Candida isolates were molecularly identified and submitted to susceptibility assays using AgNPs and commercial fungicides. The interference on biofilm formation and the mechanisms of action of AgNPs on Candida spp. were also investigated. Scanning electron microscopy was used to evaluate the morphology of AgNP-treated Candida. Candida albicans was the most frequent species isolated from denture stomatitis cases. All Candida spp. were susceptible to AgNPs at low concentrations, except Candida parapsilosis. AgNPs caused surface damage, cell disruption, and biofilm formation inhibition. The ergosterol supplementation protected C. albicans against the AgNP action. AgNPs are effective against Candida spp. and can be faced as a promising new therapeutic agent against oral candidiasis.