Sequential Release of Pooled siRNAs and Paclitaxel by Aptamer-Functionalized Shell–Core Nanoparticles to Overcome Paclitaxel Resistance of Prostate Cancer
Qianqian Guo, Yang Dong, Yanhua Zhang, Hao Fu, Chuanrong Chen, Liting Wang, Xupeng Yang, Ming Shen, Jian Yu, Meiwan Chen, Jiali Zhang, Yourong Duan
Abstract
with calcium phosphate (CaP) absorbing siRNAs formed the outer shell to sequentially release siRNAs and PTX, where CaP could trigger lysosomal escape to ensure that pooled siRNAs efficiently released into the cytoplasm to reverse EMT and resensitize PTX, while the PTX located in the core was subsequently released to exert the killing effect of chemotherapy to achieve the best synergistic effect. PTX/siRNAs NPs-Apt showed an enhanced tumor-targeting ability and achieved superior efficacy in the subcutaneous and orthotopic PCa tumor model with minimal side effects.
Topics & Concepts
LNCaPPaclitaxelSmall interfering RNAProstate cancerCancer researchAptamerMaterials sciencePharmacologyCancerBiologyMedicineCell cultureMolecular biologyInternal medicineTransfectionGeneticsRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesNanoparticle-Based Drug Delivery