Hydrogen/Deuterium Exchange Mass Spectrometry: Fundamentals, Limitations, and Opportunities
Lars Konermann, Pablo M. Scrosati
Abstract
) correction method can distort HDX kinetic profiles. Future data analysis strategies require a better fundamental understanding of HDX events, going beyond the classical Linderstrøm-Lang model. Combined with experiments that offer enhanced spatial resolution and suppressed back exchange, it should become possible to uncover the exact range of motions exhibited by a protein under a given set of conditions. Such advances would provide a greatly improved understanding of protein behavior in health and disease.
Topics & Concepts
Mass spectrometryHydrogen–deuterium exchangeDeuteriumChemistryHydrogenChromatographyPhysicsNuclear physicsOrganic chemistryMass Spectrometry Techniques and ApplicationsAnalytical Chemistry and ChromatographyMetabolomics and Mass Spectrometry Studies