Rational Design of <i>Meso</i> -Diaminopimelate Dehydrogenase with Enhanced Reductive Amination Activity for Efficient Production of <scp>d</scp> - <i>p</i> -Hydroxyphenylglycine
Yang Tan, Changzheng Gao, Wei Song, Wanqing Wei, Jia Liu, Cong Gao, Liang Guo, Xiulai Chen, Li Liu, Jing Wu
Abstract
d - p -hydroxyphenylglycine ( d -HPG) is an important intermediate in the synthesis of antimicrobial compounds. d -HPG is mainly produced via chemical and enzymatic approaches, and enzymatic asymmetric amination employing diaminopimelate dehydrogenase (DAPDH) is considered an attractive method.
Topics & Concepts
AminationChemistryStereochemistryDehydrogenaseEnzymeReductive aminationEnzyme kineticsCatalysisSubstrate (aquarium)Active siteCombinatorial chemistryBiochemistryBiologyEcologyEnzyme Catalysis and ImmobilizationBiochemical and Molecular ResearchEnzyme Structure and Function