5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
Luxi Chen, Wei Xu, Kunpeng Liu, Zheng Jiang, Han Yang, Hongbin Jin, Lin Zhang, Weimin Shen, Shunji Jia, Qianwen Sun, Anming Meng
Abstract
levels, respectively, and causes embryonic lethality that is efficiently rescued by coinjection of properly refolded corresponding tRNA. In zebrafish embryos, tRNA:DNA and 5′tRFl:DNA hybrids commonly exist on the template strand of tRNA genes. Mechanistically, unstable 5′tRFl:DNA hybrid may prevent the formation of transcriptionally inhibitory stable tRNA:DNA hybrids on the same tRNA loci so as to facilitate tRNA gene transcription. The uncovered mechanism may be implicated in other physiological and pathological processes.
Topics & Concepts
Transfer RNABiologyZebrafishGeneGene knockdownTranscription (linguistics)GeneticsRNAMorpholinoCell biologyLinguisticsPhilosophyRNA modifications and cancerRNA and protein synthesis mechanismsRNA Research and Splicing