Enabling chemical protein (semi)synthesis <i>via</i> reducible solubilizing tags (RSTs)
Jiamei Liu, Tongyao Wei, Yi Tan, Heng Liu, Xuechen Li
Abstract
The reducible solubilizing tag strategy served as a simple and powerful method for the chemical synthesis and semi-synthesis via Ser/Thr ligation and Cys/Pen ligation of extensive self-assembly peptides, membrane proteins with poor solubility.
Topics & Concepts
SalicylaldehydeLinkerPeptideNative chemical ligationChemistrySolubilityCombinatorial chemistryChemical ligationBiochemistryProtein tagChemical synthesisComputer scienceStereochemistryOrganic chemistryRecombinant DNAIn vitroFusion proteinOperating systemGeneSchiff baseChemical Synthesis and AnalysisPeptidase Inhibition and AnalysisClick Chemistry and Applications